Enumeration of Coliforms and E.coli

Enumeration of Coliforms and E. coli Objective The objective of this experiment was to comp ar and furrow several(prenominal) different enumeration methods, which were utilise to determine the approximate microbial populations in foods. The enumeration methods that ar performed in this experiment are the spread home office, Petrifilm habitation, and spiral coat for aerobic collection plate count, coliform, Petrifilm violet Red Bile (VRB), and most probable takings (MPN) techniques. Materials and Methods revel refer to the FDST (MIBO) 4030 Lab Manual for the materials and methods used for this experiment1. Results put over 1 aerophilic plate MethodColiform Forming unit (cfu/mL) Spiral Plate2.20E3 mussiness Plate1.80E4 Petrifilm Plate2.01E4 When plotting the data for the Aerobic plate methods, that screwing be seen in Table 1, we discovered that the results chain of mountains from 2.20E3 dependance forming units/mL to 2.01E4 resolving power for ming units/mL, which is a difference of almost 10 folds. The method with the least colony forming units per mL is the spiral plate method with 2.2E3, followed by the spread plate with 1.8E4, and lastly the Petrifilm plate method with the superlative count of 2.01E4. Table 2 Coliform Method colonization Forming Unit (cfu/mL) Solid Medium/Pour plate1.40E2 Petrifilm VRB2.00E2 MPN2.

10E2 The results for the coliform methods as seen above in Table 2 show that the colony forming units per mL do not vary as much; they only range from 1.40E2 to 2.10E2. The solid medium pour plate method has the low count with 1.4 0E2, followed by the Petrifilm VRB, with 2.0! E2, and last with the highest count, the MPN (Most seeming Number) method with 2.1E2. reciprocation When using the aerobic plate methods, our group used the 102 dilution for the spread plate and Petrifilm plate and one C dilution for the spiral plate method due to the fact that they crazy into the suitable colony counting range of 25 to 250. by and by converting our colony counts into the colony forming units/mL our results show,...If you want to perish a full essay, order it on our website: OrderCustomPaper.com

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